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metamorph isosurface function  (MetaMorph Inc)

 
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    MetaMorph Inc metamorph isosurface function
    (A) A cartoon representing the number of intestinal cells and the approximate number of total cells in the stages scored throughout this study. (B) Large extrachromosomal arrays containing numerous lacO sites represents a pseudo chromosome that when bound by LacI::GFP is used to visualize chromatin morphology. Intestinal nuclei were scored as open or closed based on array morphology . L4 hermaphrodites were placed at 20°C or 26°C and mixed stage F1 embryos were collected and percentage of intestinal nuclei with open arrays at the 8E, 16E, comma, and L1 stage at 20°C and 26°C were scored. 2-way ANOVA was used to determine significance: Asterisks represent significant difference between the wild-type population at that stage and temperature (p<0.01). Error bars indicate standard error of the proportion. n>100 cells (C) 3-D volumetric chromatin compaction analysis was performed using the <t>isosurface</t> function in Metamorph. The volume of the nucleus and the array were measured and used to calculate the percentage of the nucleus that the array encompassed. The percentage of the nucleus contained by the array was plotted at 8E, 16E, and comma at 20°C and 26°C. Each dot represents a single intestinal cell and the line represents the median. Asterisk represents a significant difference between 20°C and 26°C (2-way Anova p<0.01).
    Metamorph Isosurface Function, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/metamorph isosurface function/product/MetaMorph Inc
    Average 90 stars, based on 1 article reviews
    metamorph isosurface function - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "C. elegans synMuv B proteins regulate spatial and temporal chromatin compaction during development"

    Article Title: C. elegans synMuv B proteins regulate spatial and temporal chromatin compaction during development

    Journal: bioRxiv

    doi: 10.1101/538801

    (A) A cartoon representing the number of intestinal cells and the approximate number of total cells in the stages scored throughout this study. (B) Large extrachromosomal arrays containing numerous lacO sites represents a pseudo chromosome that when bound by LacI::GFP is used to visualize chromatin morphology. Intestinal nuclei were scored as open or closed based on array morphology . L4 hermaphrodites were placed at 20°C or 26°C and mixed stage F1 embryos were collected and percentage of intestinal nuclei with open arrays at the 8E, 16E, comma, and L1 stage at 20°C and 26°C were scored. 2-way ANOVA was used to determine significance: Asterisks represent significant difference between the wild-type population at that stage and temperature (p<0.01). Error bars indicate standard error of the proportion. n>100 cells (C) 3-D volumetric chromatin compaction analysis was performed using the isosurface function in Metamorph. The volume of the nucleus and the array were measured and used to calculate the percentage of the nucleus that the array encompassed. The percentage of the nucleus contained by the array was plotted at 8E, 16E, and comma at 20°C and 26°C. Each dot represents a single intestinal cell and the line represents the median. Asterisk represents a significant difference between 20°C and 26°C (2-way Anova p<0.01).
    Figure Legend Snippet: (A) A cartoon representing the number of intestinal cells and the approximate number of total cells in the stages scored throughout this study. (B) Large extrachromosomal arrays containing numerous lacO sites represents a pseudo chromosome that when bound by LacI::GFP is used to visualize chromatin morphology. Intestinal nuclei were scored as open or closed based on array morphology . L4 hermaphrodites were placed at 20°C or 26°C and mixed stage F1 embryos were collected and percentage of intestinal nuclei with open arrays at the 8E, 16E, comma, and L1 stage at 20°C and 26°C were scored. 2-way ANOVA was used to determine significance: Asterisks represent significant difference between the wild-type population at that stage and temperature (p<0.01). Error bars indicate standard error of the proportion. n>100 cells (C) 3-D volumetric chromatin compaction analysis was performed using the isosurface function in Metamorph. The volume of the nucleus and the array were measured and used to calculate the percentage of the nucleus that the array encompassed. The percentage of the nucleus contained by the array was plotted at 8E, 16E, and comma at 20°C and 26°C. Each dot represents a single intestinal cell and the line represents the median. Asterisk represents a significant difference between 20°C and 26°C (2-way Anova p<0.01).

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    metamorph isosurface function  (MetaMorph Inc)
    90
    MetaMorph Inc metamorph isosurface function
    (A) A cartoon representing the number of intestinal cells and the approximate number of total cells in the stages scored throughout this study. (B) Large extrachromosomal arrays containing numerous lacO sites represents a pseudo chromosome that when bound by LacI::GFP is used to visualize chromatin morphology. Intestinal nuclei were scored as open or closed based on array morphology . L4 hermaphrodites were placed at 20°C or 26°C and mixed stage F1 embryos were collected and percentage of intestinal nuclei with open arrays at the 8E, 16E, comma, and L1 stage at 20°C and 26°C were scored. 2-way ANOVA was used to determine significance: Asterisks represent significant difference between the wild-type population at that stage and temperature (p<0.01). Error bars indicate standard error of the proportion. n>100 cells (C) 3-D volumetric chromatin compaction analysis was performed using the <t>isosurface</t> function in Metamorph. The volume of the nucleus and the array were measured and used to calculate the percentage of the nucleus that the array encompassed. The percentage of the nucleus contained by the array was plotted at 8E, 16E, and comma at 20°C and 26°C. Each dot represents a single intestinal cell and the line represents the median. Asterisk represents a significant difference between 20°C and 26°C (2-way Anova p<0.01).
    Metamorph Isosurface Function, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/metamorph isosurface function/product/MetaMorph Inc
    Average 90 stars, based on 1 article reviews
    metamorph isosurface function - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) A cartoon representing the number of intestinal cells and the approximate number of total cells in the stages scored throughout this study. (B) Large extrachromosomal arrays containing numerous lacO sites represents a pseudo chromosome that when bound by LacI::GFP is used to visualize chromatin morphology. Intestinal nuclei were scored as open or closed based on array morphology . L4 hermaphrodites were placed at 20°C or 26°C and mixed stage F1 embryos were collected and percentage of intestinal nuclei with open arrays at the 8E, 16E, comma, and L1 stage at 20°C and 26°C were scored. 2-way ANOVA was used to determine significance: Asterisks represent significant difference between the wild-type population at that stage and temperature (p<0.01). Error bars indicate standard error of the proportion. n>100 cells (C) 3-D volumetric chromatin compaction analysis was performed using the isosurface function in Metamorph. The volume of the nucleus and the array were measured and used to calculate the percentage of the nucleus that the array encompassed. The percentage of the nucleus contained by the array was plotted at 8E, 16E, and comma at 20°C and 26°C. Each dot represents a single intestinal cell and the line represents the median. Asterisk represents a significant difference between 20°C and 26°C (2-way Anova p<0.01).

    Journal: bioRxiv

    Article Title: C. elegans synMuv B proteins regulate spatial and temporal chromatin compaction during development

    doi: 10.1101/538801

    Figure Lengend Snippet: (A) A cartoon representing the number of intestinal cells and the approximate number of total cells in the stages scored throughout this study. (B) Large extrachromosomal arrays containing numerous lacO sites represents a pseudo chromosome that when bound by LacI::GFP is used to visualize chromatin morphology. Intestinal nuclei were scored as open or closed based on array morphology . L4 hermaphrodites were placed at 20°C or 26°C and mixed stage F1 embryos were collected and percentage of intestinal nuclei with open arrays at the 8E, 16E, comma, and L1 stage at 20°C and 26°C were scored. 2-way ANOVA was used to determine significance: Asterisks represent significant difference between the wild-type population at that stage and temperature (p<0.01). Error bars indicate standard error of the proportion. n>100 cells (C) 3-D volumetric chromatin compaction analysis was performed using the isosurface function in Metamorph. The volume of the nucleus and the array were measured and used to calculate the percentage of the nucleus that the array encompassed. The percentage of the nucleus contained by the array was plotted at 8E, 16E, and comma at 20°C and 26°C. Each dot represents a single intestinal cell and the line represents the median. Asterisk represents a significant difference between 20°C and 26°C (2-way Anova p<0.01).

    Article Snippet: Images were captured on Nikon Confocal and analyzed using Metamorph isosurface function to determine the 3-D distance between the two centroids as described in .

    Techniques: